Composite

Part:BBa_K4297052

Designed by: Yang Gu   Group: iGEM22_NJXDF-CHN   (2022-10-10)


PYLXP'-ARO9

The plasmid pYLXP' contains the expressiom cassette of transaminase ARO9, which can catalyze the reaction of L-phenylalanine to phenylpyruvate.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1293
    Illegal NotI site found at 1448
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 249
    Illegal BamHI site found at 5254
    Illegal BamHI site found at 6039
    Illegal BamHI site found at 6110
    Illegal XhoI site found at 4316
    Illegal XhoI site found at 4349
    Illegal XhoI site found at 4950
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 950
    Illegal AgeI site found at 4585
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1
    Illegal BsaI site found at 1284
    Illegal BsaI site found at 4588
    Illegal BsaI site found at 4642
    Illegal BsaI site found at 5244
    Illegal BsaI.rc site found at 255
    Illegal BsaI.rc site found at 4582
    Illegal BsaI.rc site found at 5128
    Illegal BsaI.rc site found at 6203
    Illegal SapI.rc site found at 6060


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